Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation. The ideal anti-OA drug should have immunomodulatory effects while at the same time having limited or no toxicity. We examined the anti-inflammatory effects of Ginkgo biloba extract (EGb) in interleukin-1 (IL-1)-stimulated human chondrocytes. Chondrocytes were prepared from cartilage specimens taken from patients with osteoarthritis who had received total hip or total knee replacement. The concentrations of chemokines and the degree of cell migration were determined by ELISA and chemotaxis assays, respectively. The activation of inducible nitric oxide synthase (iNOS), mitogen-activated protein kinases (MAPKs), activator protein-1 (AP-1), and nuclear factor-kappaB (NF-κB) was determined by immunoblotting, immunohistochemistry, and electrophoretic mobility shift assay. We found that EGb inhibited IL-1-induced production of chemokines, which in turn resulted in attenuation of THP-1 cell migration toward EGb-treated cell culture medium. EGb also suppressed IL-1-stimulated iNOS expression and release of nitric oxide (NO). The EGb-mediated suppression of the iNOS-NO pathway correlated with the attenuation of activator protein-1 (AP-1) but not nuclear factor-kappaB (NF-κB) DNA-binding activity. Of the mitogen-activated protein kinases (MAPKs), EGb inhibited only c-Jun N-terminal kinase (JNK). Unexpectedly, EGb selectively caused degradation of c-Jun protein. Further investigation revealed that EGb-mediated c-Jun degradation was preceded by ubiquitination of c-Jun and could be prevented by the proteosome inhibitor MG-132. The results imply that EGb protects against chondrocyte degeneration by inhibiting JNK activation and inducing ubiquitination-dependent c-Jun degradation... Our results suggest that Gingko Biloba extract is a therapeutic agent for the treatment of OA. Steplex is designed to address the drawbacks of most typical joint remedies. Typical joint remedies Steplex Pills can have a damaging effect on internal organs Acts locally, directly at the site of the disease Pain relievers only temporarily relieve pain Helps to eliminate pain completely Creams with an aggressive effect can cause burns and irritation of the skin Cares for the skin thanks to the effect of emollient ingredients Chondroprotectors must be taken for six months to a year Course of admission - 30 days. Steplex is a gel that provides rapid pain relief. It contains ginkgo biloba to strengthen blood vessel walls, stimulate blood circulation, and quickly reduce inflammation and edema. Caffeine significantly increases the activity of other functional substances, removing edema. Capsaicin quickly relieves pain by affecting nerve receptors, eliminating spasms and edema. Menthol produces an anesthetic effect and improves the penetration of active substances into the skin. Citral is an organic compound with analgesic, anti-inflammatory, and antiseptic effects. Chestnut seed extract improves local blood circulation in the joint area, reducing inflammation and pain. The research collective of five scientific institutes and medical centers conducted a study in 2014 showing that Ginkgo Biloba reduces joint inflammation, modulates local immunity, and protects against degeneration of chondrocytes - the main cells of cartilage tissue. Steplex helps eliminate pain and inflammation in cases of bumps, radiculitis, osteochondrosis, other diseases, and injuries of the spine.